Oct 2019 - present
Designing an in vitro model which mimics melasma condition through UV and hormone combination
Evaluating the role of skin microbes on host skin pigmentation
Screening novel gene targets from distinct melanisation pathways for melasma.
Evaluating compounds for treatment of Hyper-pigmentation disorders using co-culture technique.
Comparative analysis of genes and its variation associated with pigmentation using in silico studies.
Generating scientific insights for microbiome-based claims for beauty and personal care products
Developing novel in vitro models to study host microbe interaction in skin
Evaluating antimicrobial efficacy of personal hygiene products
Evaluating the efficacy of fast acting antimicrobials for germkill benefits in formats like soap, handwash etc. (ASTM standard)
Planning and execution of human intervention trails for ex vivo germ kill benefit. (ASTM standard)
Providing technical insights for claims support for various skin care and cleansing brands using 3D skin models
Communicating the findings as internal reports to stakeholders
Dec 2018 - Oct 2019 (9 months)
Project 1: Role of GPCRs in host and microbe interaction on skin.
Established the profile of GPCRSon human skin. The role of various GPCR sinmediating skin-microbe crosstalk was elucidated. Identified new targets for improving and strengthening epidermal barrier and improved moisturization.
Independent handling of 3D skin, primary cells, and established cell lines (HaCaT, THP-1, Vaginal epithelial cells).
Technical acumen in RNA isolation, qPCR technique, and ELISA.
Highly skilled in developing various cell-based bioassays (Cytokines, AMPs etc)
Well versed in microbiology techniques like growth curve analysis, plating, PCR based molecular characterization of microbes
Project 2: Evaluation of skin care/cleansing actives for barrier boosting benefits
Evaluated skin cleansing and skin care actives for improving tight junction proteins using immunofluorescence.
Proficient in Immunostaining (Fluorescence Microscopy) and analysis of epidermal markers like (Claudin, Filaggrin, Desmoglin, Cytokeratin) and receptors